Ensemble of nucleic acid absolute quantitation modules for copy number variation detection and RNA profiling
Highly multiplexed ddPCR for sensitive nucleic acid quantitation remains challenging due to limited fluorescence channels. Here the authors report quantitative amplicon sequencing (QASeq), a PCR-based molecular barcoding NGS approach which is compatible with high multiplexing.
Bibliographic Details
| Published in: | Nature Communications |
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| Main Authors: |
Lucia Ruojia Wu,
Peng Dai,
Michael Xiangjiang Wang,
Sherry Xi Chen,
Evan N. Cohen,
Gitanjali Jayachandran,
Jinny Xuemeng Zhang,
Angela V. Serrano,
Nina Guanyi Xie,
Naoto T. Ueno,
James M. Reuben,
Carlos H. Barcenas,
David Yu Zhang |
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| Format: | Article
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| Language: | English |
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| Published: |
Nature Portfolio
2022-04-01
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| Online Access: | https://doi.org/10.1038/s41467-022-29487-y
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