Optimization of the cytogenetic protocol for Pangasianodon hypophthalmus (Sauvage, 1878) and Clarias gariepinus (Burchell, 1822)

To obtain well spread chromosomes, the cytogenetic protocol for Pangasianodon hypophthalmus and Clarias gariepinus were optimized. This includes, the colchicine concentration (0.01%, 0.025%, 0.05%)/exposure duration (1, 3, and 5 h), hypotonic solution (distilled water or 0.075M KCl solution)/exposur...

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Bibliographic Details

Published in:	PeerJ
Main Authors:	Victor T. Okomoda, Ivan C.C. Koh, Anuar Hassan, Thumronk Amornsakun, Julia H.Z. Moh, Sheriff Md Shahreza
Format:	Article
Language:	English
Published:	PeerJ Inc. 2018-11-01
Subjects:	African catfish Metaphase Colchicine Asian catfish Giemsa stain
Online Access:	https://peerj.com/articles/5712.pdf