Protocol to study projection-specific circuits in the basal ganglia of adult mice using viral vector tracing, optogenetics, and patch-clamp technique

• الحاوية / القاعدة: STAR Protocols
• المؤلفون الرئيسيون: Ya-Wei Ji, Xiang-Ying Xu, Cui Yin, Chunyi Zhou, Cheng Xiao
• التنسيق: مقال
• اللغة: الإنجليزية
• منشور في: Elsevier 2023-09-01
• الموضوعات: Model Organisms; Molecular Biology; Neuroscience; Molecular/Chemical Probes
• الوصول للمادة أونلاين: http://www.sciencedirect.com/science/article/pii/S266616672300518X
• تدمد: 2666-1667

Summary: Analysis of synaptic strength and plasticity provides functional insights of complicated neural circuits. Here, we describe steps for cell- and projection-specific optogenetic manipulation of divergent basal ganglia circuits using anterograde and retrograde viral vectors. We quantitatively analyze synaptic function of these circuits utilizing a patch-clamp technique. This protocol is applicable to probe potential circuit targets for treatment of brain diseases.For complete details on the use and execution of this protocol, please refer to Ji et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.