Label-free assessment of complement-dependent cytotoxicity of therapeutic antibodies via a whole-cell MALDI mass spectrometry bioassay

Abstract Potency assessment of monoclonal antibodies or corresponding biosimilars in cell-based assays is an essential prerequisite in biopharmaceutical research and development. However, cellular bioassays are still subject to limitations in sample throughput, speed, and often need costly reagents...

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Bibliographic Details

Published in:	Scientific Reports
Main Authors:	Stefan Schmidt, Alexander Geisel, Thomas Enzlein, Björn C. Fröhlich, Louise Pritchett, Melanie Verneret, Christian Graf, Carsten Hopf
Format:	Article
Language:	English
Published:	Nature Portfolio 2024-09-01
Subjects:	MALDI Mass spectrometry Complement-dependent cytotoxicity Monoclonal antibody Label-free cell assay Drug-response curves
Online Access:	https://doi.org/10.1038/s41598-024-71483-3