Analysis of essential genes in Clostridioides difficile by CRISPRi and Tn-seq

ABSTRACT Essential genes are interesting in their own right and as potential antibiotic targets. To date, only one report has identified essential genes on a genome-wide scale in Clostridioides difficile, a problematic pathogen for which treatment options are limited. That foundational study used la...

Full description

Bibliographic Details

Published in:	Journal of Bacteriology
Main Authors:	Maia E. Alberts, Micaila P. Kurtz, Ute Müh, Jonathon P. Bernardi, Kevin W. Bollinger, Horia A. Dobrila, Leonard Duncan, Hannah M. Laster, Andres J. Orea, Anthony G. Pannullo, Juan G. Rivera-Rosado, Facundo V. Torres, Craig D. Ellermeier, David S. Weiss
Format:	Article
Language:	English
Published:	American Society for Microbiology 2025-10-01
Subjects:	CRISPRi Tn-seq
Online Access:	https://journals.asm.org/doi/10.1128/jb.00220-25