Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in Saccharomyces cerevisiae

Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters. Fluorescent proteins must undergo in vivo maturation after expression before they become photoactive. Maturation effects must be q...

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Bibliographic Details

Published in:	Bio-Protocol
Main Authors:	Sviatlana Shashkova, Adam Wollman, Stefan Hohmann, Mark C Leake
Format:	Article
Language:	English
Published:	Bio-protocol LLC 2018-01-01
Online Access:	https://bio-protocol.org/en/bpdetail?id=2710&type=0