Straightforward Synthesis of BHQ-3 Amine: An Azo Dark-Quencher for FRET-Based Protease Activity Assays

A Black Hole Quencher-3 (BHQ-3) derivative was synthesized through an azo-coupling reaction between Methylene Violet 3RAX and a tertiary aniline functionalized with a pendant primary amine, allowing subsequent peptide conjugation. The synthesized compounds were characterized using NMR, UV–vis absorp...

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Bibliographic Details

Published in:	Chemistry Proceedings
Main Authors:	Cátia D. F. Martins, Maria Manuela M. Raposo, Susana P. G. Costa
Format:	Article
Language:	English
Published:	MDPI AG 2024-11-01
Subjects:	azo dyes BHQ-3 FRET proteases quenching
Online Access:	https://www.mdpi.com/2673-4583/16/1/48