Expression of Tailored α-N-Acetylglucosaminidase in <i>Escherichia coli</i> for Synthesizing Mannose-6-Phosphate on N-Linked Oligosaccharides of Lysosomal Enzymes

Lysosomal enzymes are synthesized as N-glycosylated glycoproteins with mannose-6-phosphate (M6P) moieties, which are responsible for their binding to M6P receptors and transporting to the lysosome. In the M6P biosynthetic pathway, a Man₈GlcNAc₂ glycoform...

Full description

Bibliographic Details

Published in:	Bioengineering
Main Authors:	Yunsong Cao, Wei Wang
Format:	Article
Language:	English
Published:	MDPI AG 2025-04-01
Subjects:	lysosomal enzyme uncovering enzyme mannose-6-phosphate maltose-binding protein (MBP)
Online Access:	https://www.mdpi.com/2306-5354/12/4/425