Incorporation of measurement of DNA integrity into qPCR assays
Optimal accuracy of quantitative PCR (qPCR) requires correction for integrity of the target sequence. Here we combine the mathematics of the Poisson distribution and exponential amplification to show that the frequency of lesions per base (which prevent PCR amplification) can be derived from the slo...
| Published in: | BioTechniques |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2010-12-01
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| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/000113567 |