Protocol for simplified parallel perturbations using an abridged long non-coding RNA CRISPR library

Summary: High-throughput CRISPR interference (CRISPRi) screens are invaluable for discovering novel functional genes, but applying such screens to long non-coding RNAs (lncRNAs) is more challenging. Here, we present a protocol for designing and executing pooled CRISPRi screens targeting lncRNAs usin...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Joshua M. Hazan, Nisrine Lahoud-Jeries, Assaf C. Bester
Format:	Article
Language:	English
Published:	Elsevier 2025-12-01
Subjects:	Cell Biology Genetics Gene Expression CRISPR
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166725005167