Enhancing single-cell transcriptomics using interposed anchor oligonucleotide sequences

Abstract Single-cell transcriptomics, which utilises barcodes and unique molecular identifiers (UMIs) for polyA+ mRNA capture, is compromised by oligonucleotide synthesis errors. To address this, we modified the oligonucleotide capture design and integrated an interposed anchor between the barcode a...

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出版年:	Communications Biology
主要な著者:	Jianfeng Sun, Martin Philpott, Danson Loi, Gabriela Hoffman, Jonathan Robson, Neelam Mehta, Eleanor Calcutt, Vicki Gamble, Tom Brown, Udo Oppermann, Adam P. Cribbs
フォーマット:	論文
言語:	英語
出版事項:	Nature Portfolio 2025-01-01
オンライン･アクセス:	https://doi.org/10.1038/s42003-025-07474-5