Rapid O-glycan release from glycoproteins using a monolith-based glycosidase microreactor

Fast, efficient and simple selective O-glycan release methods are urgently needed for accurately analyzing O-glycans of therapeutic proteins or glycosylation-based biomarkers. In this study, a polymeric monolith-based microreactor with immobilized O-glycosidase inside a capillary has been developed....

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Bibliographic Details

Published in:	Journal of Chromatography Open
Main Authors:	Bin Yang, Myriam Taverna, Cédric Y. Montanier, Zhengjin Jiang, N. Thuy Tran
Format:	Article
Language:	English
Published:	Elsevier 2025-11-01
Subjects:	O-glycosidase Immobilized enzyme microreactor Core 1 O-glycan Capillary electrophoresis Monolith Glycoprotein
Online Access:	http://www.sciencedirect.com/science/article/pii/S2772391725000672