PCR-based tandem epitope tagging system for Escherichia coli genome engineering

Biological discovery in the postgenomic era requires a systematic and high-throughput experimental approach. To this end, a versatile PCR-based tandem epitope tagging system is described, which inserts a tandem epitope coding sequence into any desired position of the Escherichia coli chromosome. Tem...

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Bibliographic Details
Published in:BioTechniques
Main Authors: Byung-Kwan Cho, Eric M. Knight, Bernhard Ø. Palsson
Format: Article
Language:English
Published: Taylor & Francis Group 2006-01-01
Online Access:https://www.future-science.com/doi/10.2144/000112039