Protocol to quantify desmoglein 3 tension at cell-cell adhesions using FRET-based tension sensors

Summary: Desmoglein 3 (Dsg3) is a crucial component of cell-cell junctions, contributing to mechanotransduction and maintaining tissue integrity. Here, we present a protocol to quantify tension at keratinocyte cell-cell adhesion sites using a Dsg3 fluorescence resonance energy transfer (FRET)-based...

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發表在:	STAR Protocols
Main Authors:	Bahareh Tajvidi Safa, Timothy Goldsmith, Zachary Kezhaya, Sarah Seman, Daniel E. Conway, Ruiguo Yang
格式:	Article
語言:	英语
出版:	Elsevier 2026-09-01
主題:	Biophysics Cell Biology Biotechnology and bioengineering
在線閱讀:	http://www.sciencedirect.com/science/article/pii/S2666166726002832