Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step.

The ongoing COVID-19 pandemic has created an unprecedented need for rapid diagnostic testing. The World Health Organization (WHO) recommends a standard assay that includes an RNA extraction step from a nasopharyngeal (NP) swab followed by reverse transcription-quantitative polymerase chain reaction...

Full description

Bibliographic Details

Published in:	PLoS Biology
Main Authors:	Emily A Bruce, Meei-Li Huang, Garrett A Perchetti, Scott Tighe, Pheobe Laaguiby, Jessica J Hoffman, Diana L Gerrard, Arun K Nalla, Yulun Wei, Alexander L Greninger, Sean A Diehl, David J Shirley, Debra G B Leonard, Christopher D Huston, Beth D Kirkpatrick, Julie A Dragon, Jessica W Crothers, Keith R Jerome, Jason W Botten
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2020-10-01
Online Access:	https://doi.org/10.1371/journal.pbio.3000896