Fluorescent reporters give new insights into antibiotics-induced nonsense and frameshift mistranslation

Abstract We developed a reporter system based on simultaneous expression of two fluorescent proteins: GFP as a reporter of the capacity of protein synthesis and mutated mScarlet-I as a reporter of translational errors. Because of the unique stop codons or frameshift mutations introduced into the mSc...

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Bibliographic Details
Published in:Scientific Reports
Main Authors: Mariliis Hinnu, Marta Putrinš, Karin Kogermann, Niilo Kaldalu, Tanel Tenson
Format: Article
Language:English
Published: Nature Portfolio 2024-03-01
Online Access:https://doi.org/10.1038/s41598-024-57597-8