Protocol for image-based monitoring of de novo RNA synthesis at DNA double-strand breaks in human cell lines

Summary: DNA double-strand breaks (DSBs) halt canonical transcription and simultaneously trigger local non-canonical RNA synthesis. Despite its significance, existing approaches to monitor this process are limited. Here, we present a protocol to monitor and quantify nascent transcription on DSBs in...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Georgios Pappas, Helena Hagner Gram, Jiri Bartek, Panagiotis Galanos
Format:	Article
Language:	English
Published:	Elsevier 2025-12-01
Subjects:	Cell Biology Cell culture Microscopy Molecular Biology Gene Expression
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166725005635