Recombinant PASylated nanobody probes with improved blood circulation and tumor targeting

• 出版年: Journal of Innovative Optical Health Sciences
• 主要な著者: Yicheng Yang, Lingyue Jin, You Zhang, Siyu Zhou, Weijun Wei, Gang Huang, Changfeng Wu
• フォーマット: 論文
• 言語: 英語
• 出版事項: World Scientific Publishing 2025-05-01
• 主題: Nanobody; PASylation; fluorescence; tumor targeting
• オンライン･アクセス: https://www.worldscientific.com/doi/10.1142/S1793545825410019
• ISSN: 1793-5458; 1793-7205

Nanobodies have been extensively demonstrated in biomedical imaging and therapy. However, nanobody probes often suffer from rapid renal clearance due to its small size. Herein, we reported a recombinant nanobody with a 200 amino-acid polypeptide chain consisting of Pro, Ala, and Ser (PAS) at the C-terminal, which can be easily expressed in Escherichia coli with a high yield. The PASylated nanobody was functionalized with a fluorescent dye and the cell labeling properties were characterized by flow cytometry and confocal microscopy. In vivo fluorescence imaging indicated that the PASylated nanobody showed comparable blood circulation time, but [Formula: see text] times higher tumor targeting ability as compared to the PEGylated nanobody of comparable molecular weight. Our findings demonstrate that nanobody PASylation is a promising approach to produce long-circulating nanobody probes for imaging and therapeutic applications.