Technical report: liquid overlay technique allows the generation of homogeneous osteosarcoma, glioblastoma, lung and prostate adenocarcinoma spheroids that can be used for drug cytotoxicity measurements

Introduction: The mechanisms involved in cancer initiation, progression, drug resistance, and disease recurrence are traditionally investigated through in vitro adherent monolayer (2D) cell models. However, solid malignant tumor growth is characterized by progression in three dimensions (3D), and an...

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Bibliographic Details

Published in:	Frontiers in Bioengineering and Biotechnology
Main Authors:	Camille Jubelin, Javier Muñoz-Garcia, Denis Cochonneau, Emilie Ollivier, François Vallette, Marie-Françoise Heymann, Lisa Oliver, Dominique Heymann
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2023-10-01
Subjects:	3D culture (three-dimensional spheroids) homogeneous spheroid model liquid overlay technique hanging drop lung adenocarcinoma prostate adenocarcinoma
Online Access:	https://www.frontiersin.org/articles/10.3389/fbioe.2023.1260049/full