A Python script to design primers for overlap extension PCR to ligate two DNA fragments

Ligating two or more DNA fragments is a regular operation for the subcloning and the engineering of vectors. The overlap extension PCR serves as a straightforward method to solve this issue. However, it takes a relatively long time to design the appropriate overlapping primers and the primers for th...

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Bibliographic Details

Published in:	PeerJ
Main Authors:	Yunzhuo Hu, Fei Xu, Bifang Huang, Xuanyang Chen, Shiqiang Lin
Format:	Article
Language:	English
Published:	PeerJ Inc. 2022-10-01
Subjects:	DNA joining Overlap extension PCR Primer design Python script
Online Access:	https://peerj.com/articles/14283.pdf