Development of alkaline phosphatase-scFv and its use for one-step enzyme-linked immunosorbent assay for His-tagged protein detection

A histidine (His)-tag is composed of six His residues and typically exerts little influence on the structure and solubility of expressed recombinant fusion proteins. Purification methods for recombinant proteins containing His-tags are relatively well-established, thus His-tags are widely used in pr...

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Bibliographic Details

Published in:	Open Life Sciences
Main Authors:	He Shuzhen, Xu Ruixian, Yi Huashan, Chen Zhixin, Chen Congjie, Li Qiang, Han Qinqin, Xia Xueshan, Song Yuzhu, Xu Junwei, Zhang Jinyang
Format:	Article
Language:	English
Published:	De Gruyter 2022-11-01
Subjects:	alkaline phosphatase direct elisa glutathione s-transferase his-tagged fusion protein one-step western blot scfv
Online Access:	https://doi.org/10.1515/biol-2022-0521