Assessing Ploidy Level Analysis and Single Pollen Genotyping of Diploid and Euploid Citrus Genotypes by Fluorescence-Activated Cell Sorting and Whole-Genome Amplification

Flow cytometry is widely used to determine genome size and ploidy level in plants. This technique, when coupled with fluorescence-activated cell sorting (FACS), whole genome amplification and genotyping (WGA), opens up new opportunities for genetic studies of individualized nuclei. This strategy was...

Full description

Bibliographic Details

Published in:	Frontiers in Plant Science
Main Authors:	Miguel Garavello, José Cuenca, Steven Dreissig, Jörg Fuchs, Andreas Houben, Pablo Aleza
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2019-09-01
Subjects:	flow cytometry triploid tetraploid fluorescence-activated cell sorting whole genome amplification SSR and SNP markers
Online Access:	https://www.frontiersin.org/article/10.3389/fpls.2019.01174/full