Tissue clearing and three-dimensional imaging of the whole cochlea and vestibular system from multiple large-animal models

Summary: The inner ear of humans and large animals is embedded in a thick and dense bone that makes dissection challenging. Here, we present a protocol that enables three-dimensional (3D) characterization of intact inner ears from large-animal models. We describe steps for decalcifying bone, using s...

Full description

Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Adele Moatti, Yuheng Cai, Chen Li, Kristen D. Popowski, Ke Cheng, Frances S. Ligler, Alon Greenbaum
Format:	Article
Language:	English
Published:	Elsevier 2023-06-01
Subjects:	Microscopy Biotechnology and Bioengineering
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723001788