When Chromatin Decondensation Affects Nuclear γH2AX Foci Pattern and Kinetics and Biases the Assessment of DNA Double-Strand Breaks by Immunofluorescence

Immunofluorescence with antibodies against phosphorylated forms of H2AX (γH2AX) is revolutionizing our understanding of repair and signaling of DNA double-strand breaks (DSBs). Unfortunately, the pattern of γH2AX foci depends upon a number of parameters (nature of stress, number of foci, radiation d...

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Bibliographic Details

Published in:	Biomolecules
Main Authors:	Adeline Granzotto, Laura El Nachef, Juliette Restier-Verlet, Laurène Sonzogni, Joëlle Al-Choboq, Michel Bourguignon, Nicolas Foray
Format:	Article
Language:	English
Published:	MDPI AG 2024-06-01
Subjects:	DNA double-strand breaks ATM immunofluorescence chromatin condensation sodium butyrate
Online Access:	https://www.mdpi.com/2218-273X/14/6/703