A targeted liquid cultivation method for previously uncultured non-colony forming microbes

A large number of microbes are not able to form colonies using agar-plating methods, which is one of the reasons that cultivation based on solid media leaves the majority of microbial diversity in the environment inaccessible. We developed a new Non-Colony-Forming Liquid Cultivation method (NCFLC) t...

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Bibliographic Details

Published in:	Frontiers in Microbiology
Main Authors:	Eun-Young Seo, Dawoon Jung, Slava S. Epstein, Weiyan Zhang, Jeffrey S. Owen, Hiroaki Baba, Akina Yamamoto, Mifuyu Harada, Yutaka Nakashimada, Setsu Kato, Yoshiteru Aoi, Shan He
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2023-06-01
Subjects:	non-colony-forming liquid cultivation dilution-to-extinction uncultured microbes microbial dark matter
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2023.1194466/full