Construction of Chromosomal In-Frame Deletion Mutants in Rhodobacter capsulatus
Markerless chromosomal in-frame deletion mutants are often used to avoid polar effects, conserve antibiotic resistance markers or make multiple mutations. This protocol capitalizes on the sacB gene (lethal in the presence of sucrose), for selection against a single cross-over event, on a suicide vec...
| Published in: | Bio-Protocol |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2013-02-01
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| Online Access: | https://bio-protocol.org/en/bpdetail?id=333&type=0 |