Development of a single transcript CRISPR/Cas9 toolkit for efficient genome editing in autotetraploid alfalfa

Alfalfa (Medicago sativa. L.) is a globally significant autotetraploid legume forage crop. However, despite its importance, establishing efficient gene editing systems for cultivated alfalfa remains a formidable challenge. In this study, we pioneered the development of a highly effective ultrasonic-...

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Bibliographic Details

Published in:	Crop Journal
Main Authors:	Haixia Zhao, Siyi Zhao, Yingping Cao, Xiping Jiang, Lijuan Zhao, Zhimeng Li, Mengqi Wang, Ruijuan Yang, Chuanen Zhou, Zhaoming Wang, Feng Yuan, Dongmei Ma, Hao Lin, Wenwen Liu, Chunxiang Fu
Format:	Article
Language:	English
Published:	KeAi Communications Co., Ltd. 2024-06-01
Subjects:	Alfalfa Gene editing CRISPR_2.0 toolkit Hairy root system Tetra-allelic homozygous mutants
Online Access:	http://www.sciencedirect.com/science/article/pii/S2214514124000813