Where single-cell transcriptomics fails T cells: The misuse of unsupervised clustering for T-cell annotationThis perspective used publicly available data (DOI: 10.5281/zenodo.12569981; ).

The current state of single-cell transcriptomic interrogation typically consists of using an unsupervised clustering approach followed by expert opinion-based annotation. The underlying assumption is that this process will identify transcriptional differences between cellular subsets accurately, and...

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Bibliographic Details

Published in:	ImmunoInformatics
Main Authors:	Kerry A. Mullan, Sebastiaan Valkiers, Nicky de Vrij, Chen Li, Sara Verbandt, Ting Pu, Pieter Meysman
Format:	Article
Language:	English
Published:	Elsevier 2025-12-01
Subjects:	Single-cell RNA-see T cell UMAP scRNAseq TCR
Online Access:	http://www.sciencedirect.com/science/article/pii/S2667119025000163