Efficient shRNA-based knockdown of multiple target genes for cell therapy using a chimeric miRNA cluster platform

Genome engineering technologies are powerful tools in cell-based immunotherapy to optimize or fine-tune cell functionalities. However, their use for multiple gene edits poses relevant biological and technical challenges. Short hairpin RNA (shRNA)-based cell engineering bypasses these criticalities a...

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Bibliographic Details

Published in:	Molecular Therapy: Nucleic Acids
Main Authors:	Matteo Rossi, Mikhail Steklov, Fanny Huberty, Thuy Nguyen, Jérôme Marijsse, Céline Jacques-Hespel, Paul Najm, Caroline Lonez, Eytan Breman
Format:	Article
Language:	English
Published:	Elsevier 2023-12-01
Subjects:	MT: Oligonucleotides: Therapies and Applications miRNA shRNA multiplex platform gene expression modulation
Online Access:	http://www.sciencedirect.com/science/article/pii/S2162253123002561