Sequential Accumulation of ‘Driver’ Pathway Mutations Induces the Upregulation of Hydrogen-Sulfide-Producing Enzymes in Human Colonic Epithelial Cell Organoids

Recently, a CRISPR-Cas9 genome-editing system was developed with introduced sequential ‘driver’ mutations in the WNT, MAPK, TGF-β, TP53 and PI3K pathways into organoids derived from normal human intestinal epithelial cells. Prior studies have demonstrated that isogenic organoids harboring mutations...

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Bibliographic Details

Published in:	Antioxidants
Main Authors:	Kelly Ascenção, Nahzli Dilek, Karim Zuhra, Katalin Módis, Toshiro Sato, Csaba Szabo
Format:	Article
Language:	English
Published:	MDPI AG 2022-09-01
Subjects:	Vogelstein sequence colon cancer hydrogen sulfide cystathionine beta-synthase 3-mercaptopyruvate sulfurtransferase
Online Access:	https://www.mdpi.com/2076-3921/11/9/1823