Efficient traceless modification of the P1 bacteriophage genome through homologous recombination with enrichment in double recombinants: A new perspective on the functional annotation of uncharacterized phage genes

The advent of high-throughput omic technologies has caused unprecedented progress in research on bacteriophages, the most abundant and still the least explored entities on earth. Despite the growing number of phage genomes sequenced and the rejuvenation of interest in phage therapy, the progress in...

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Bibliographic Details

Published in:	Frontiers in Microbiology
Main Authors:	Agnieszka Bednarek, Katarzyna Giermasińska-Buczek, Małgorzata Łobocka
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2023-03-01
Subjects:	bacteriophage P1 temperate bacteriophage prophage bacteriophage engineering targeted mutagenesis homologous recombination
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2023.1135870/full