3D exploration of gene expression in chicken embryos through combined RNA fluorescence in situ hybridization, immunofluorescence, and clearing

Abstract Background Fine characterization of gene expression patterns is crucial to understand many aspects of embryonic development. The chicken embryo is a well-established and valuable animal model for developmental biology. The period spanning from the third to sixth embryonic days (E3 to E6) is...

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Bibliographic Details

Published in:	BMC Biology
Main Authors:	Maëlys André, Sarah Dinvaut, Valérie Castellani, Julien Falk
Format:	Article
Language:	English
Published:	BMC 2024-06-01
Subjects:	RNA fluorescence in situ hybridization (HCR RNA-FISH) Immunofluorescence (IF) Tissue clearing ECi Whole mount chicken embryos Development
Online Access:	https://doi.org/10.1186/s12915-024-01922-0