A Robust Expression and Purification Method for Production of <i>Sp</i>Cas9-GFP-MBP Fusion Protein for In Vitro Applications

Genome editing using the CRISPR/Cas9 system is one of the trendiest methodologies in the scientific community. Many genome editing approaches require recombinant <i>Streptococcus pyogenes</i> Cas9 (<i>Sp</i>Cas9) at some point during their application, for instance, for in vi...

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Bibliographic Details

Published in:	Methods and Protocols
Main Authors:	Andrea Luciana Fleitas, Mario Señorale, Sabina Vidal
Format:	Article
Language:	English
Published:	MDPI AG 2022-05-01
Subjects:	CIRSPR/Cas9 expression purification immobilized metal affinity chromatography ion exchange
Online Access:	https://www.mdpi.com/2409-9279/5/3/44