Development of an Enzyme-Linked Immunosorbent Assay for Dibutyl Phthalate in Liquor

A monoclonal antibody specifically recognizing dibutyl phthalate (DBP) was prepared based on a hapten (di-n-butyl-4-aminophthalate). After optimizing various parameters such as concentrations of antibody, coating antigen and composition of the assay buffer, an inhibition curve was plotted with the 5...

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Bibliographic Details

Published in:	Sensors
Main Authors:	Libing Wang, Lingling Guo, Wei Ma, Liguang Xu, Liqiang Liu, Hua Kuang, Chuanlai Xu
Format:	Article
Language:	English
Published:	MDPI AG 2013-06-01
Subjects:	di-n-butyl phthalate ELISA liquor monoclonal antibody
Online Access:	http://www.mdpi.com/1424-8220/13/7/8331