Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice

Summary: Mouse hyperglycemia model and islet function assessment are essential in diabetes research. Here, we provide a protocol to evaluate glucose homeostasis and islet functions in diabetic mice and isolated islets. We describe steps for establishing type 1 and 2 diabetes, glucose tolerance test,...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Hao Chen, Jian Liu, Guo-Ping Shi, Xian Zhang
Format:	Article
Language:	English
Published:	Elsevier 2023-03-01
Subjects:	Cell Biology Cell Isolation Flow Cytometry/Mass Cytometry Developmental Biology Health Sciences Metabolism
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723000916