Improvement of optimum pH and specific activity of pectate lyase from Bacillus RN.1 using loop replacement

Background: Alkaline pectate lyase plays an important role in papermaking, biological refining and wastewater treatment, but its industrial applications are largely limited owing to its low activity and poor alkali resistance.Methods: The alkaline pectate lyase BspPel from Bacillus RN.1 was heterolo...

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Bibliographic Details

Published in:	Frontiers in Bioengineering and Biotechnology
Main Authors:	Piwu Li, Xiaofeng Wei, Yun Wang, Hui Liu, Yanpeng Xu, Ziyang Zhang, Junlin Li, Jianbin Wang, Chuanzhuang Guo, Songsen Sui, Junqing Wang, Ruiming Wang
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2023-07-01
Subjects:	pectate lyases alkali resistance loop replacement enzyme activity molecular dynamics simulation
Online Access:	https://www.frontiersin.org/articles/10.3389/fbioe.2023.1242123/full