Engineering of SauriCas9 with enhanced specificity
SauriCas9 is a compact Cas9 nuclease showing promise for in vivo therapeutic applications. However, concerns about off-target effects necessitated improvements in specificity. We addressed this by introducing mutations to eliminate polar contacts between Cas9 and the target DNA, resulting in the Sau...
| Published in: | Molecular Therapy: Nucleic Acids |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
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| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2162253125000095 |