A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry
Summary: Here, we provide a protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution. By combining tissue clearing and click chemistry, this protocol allows fluorescence tagging of covalent drug binding in situ. In addition, the protocol is compatible with m...
| 發表在: | STAR Protocols |
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| Main Authors: | , |
| 格式: | Article |
| 語言: | 英语 |
| 出版: |
Elsevier
2022-12-01
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| 主題: | |
| 在線閱讀: | http://www.sciencedirect.com/science/article/pii/S266616672200658X |