Quantification of DNase type I ends, DNase type II ends, and modified bases using fluorescently labeled ddUTP, terminal deoxynucleotidyl transferase, and formamidopyrimidine-DNA glycosylase

Here we describe the substitution of fluorescently labeled ddUTP for dUTP in the TUNEL assay to allow quantification of generated fluorescence signals by epifluorescence microscopy. The capping of DNase type I 3′OH DNA ends using ddTUNEL was further combined with phosphatase treatment for detection...

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Bibliographic Details

Published in:	BioTechniques
Main Authors:	David S. Baskin, Marsha A. Widmayer, Martyn A. Sharpe
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2010-07-01
Subjects:	TUNEL ddUTP formamidopyrimidine-DNA glycosylase DNase apoptosis mammary gland
Online Access:	https://www.future-science.com/doi/10.2144/000113439