Quantitation of DNA by nuclease P1 digestion and UPLC-MS/MS to assess binding efficiency of pyrrolobenzodiazepine

Accurate DNA quantitation is a prerequisite in many biomedical and pharmaceutical studies. Here we established a new DNA quantitation method by nuclease P1 digestion and UPLC-MS/MS analysis. DNA fragments can be efficiently hydrolyzed to single deoxyribonucleotides by nuclease P1 in a short time. Th...

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Bibliographic Details

Published in:	Journal of Pharmaceutical Analysis
Main Authors:	Yong Ma, Buyun Chen, Donglu Zhang
Format:	Article
Language:	English
Published:	Elsevier 2020-06-01
Subjects:	Nuclease P1 UPLC-MS/MS DNA quantitation DNA alkylation Pyrrolobenzodiazepine (PBD-Dimer)
Online Access:	http://www.sciencedirect.com/science/article/pii/S2095177920303981