High efficiency transfection of embryonic limb mesenchyme with plasmid DNA using square wave pulse electroporation and sucrose buffer

Micromass cultures of primary embryonic limb mesenchyme are a valuable model system for studying cartilage formation in vitro. However, high efficiency introduction of plasmid DNA into this hard-to-transfect cell type typically results in considerable cell death and significantly impeded chondrogene...

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Bibliographic Details

Published in:	BioTechniques
Main Authors:	Brent E. Bobick, Peter G. Alexander, Rocky S. Tuan
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2014-02-01
Subjects:	transfection protocol square wave pulse electroporation sucrose buffer cell suspension embryonic limb mesenchyme micromass culture
Online Access:	https://www.future-science.com/doi/10.2144/000114136