Competitive Oligonucleotide Single-Base Extension Combined with Mass Spectrometric Detection for Mutation Screening
A rapid, robust and widely applicable mutation detection scheme not requiring radioactivity or gel-based detection is introduced. It is a single-tube, competitive oligonucleotide single-base extension (COSBE) reaction using a pair of primers with the 3′-terminal base complementary to either the norm...
| Published in: | BioTechniques |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1997-10-01
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| Online Access: | https://www.future-science.com/doi/10.2144/97234st05 |