A versatile enhanced freeze-substitution protocol for volume electron microscopy

Volume electron microscopy, a powerful approach to generate large three-dimensional cell and tissue volumes at electron microscopy resolutions, is rapidly becoming a routine tool for understanding fundamental and applied biological questions. One of the enabling factors for its adoption has been the...

Full description

Bibliographic Details

Published in:	Frontiers in Cell and Developmental Biology
Main Authors:	Sébastien Bélanger, Heather Berensmann, Valentina Baena, Keith Duncan, Blake C. Meyers, Kedar Narayan, Kirk J. Czymmek
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2022-08-01
Subjects:	freeze-substitution fixation volume electron microscopy (vEM) OTO C. elegans Saccharomyces cerevisiae plant specimens
Online Access:	https://www.frontiersin.org/articles/10.3389/fcell.2022.933376/full