An improved method for large-scale preparation of negatively and positively supercoiled plasmid DNA

A rigorous understanding of the biological function of superhelical tension in cellular DNA requires the development of new tools and model systems for study. To this end, an ethidium bromide–free method has been developed to prepare large quantities of either negatively or positively super-coiled p...

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Bibliographic Details

Published in:	BioTechniques
Main Authors:	Marita C. Barth, Debra A. Dederich, Peter C. Dedon
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2009-07-01
Subjects:	DNA supercoiling rHMfB archaeal histone positive supercoiling negative supercoiling
Online Access:	https://www.future-science.com/doi/10.2144/000113172