Serial Cryomicrotomy of Saccharomyces cerevisiae for Serial Electron Cryotomography

Electron cryotomography (cryo-ET) is an increasingly popular technique to study cellular structures and macromolecules in situ. Due to poor penetration of electrons through thick biological samples, the vitreously frozen samples for cryo-ET need to be thin. For frozen-hydrated cells, such samples ca...

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Bibliographic Details
Published in:Bio-Protocol
Main Authors: Cai Tong Ng, Mark Ladinsky, Lu Gan
Format: Article
Language:English
Published: Bio-protocol LLC 2020-11-01
Online Access:https://bio-protocol.org/en/bpdetail?id=3831&type=0