Genome editing in the edible fungus Poria cocos using CRISPR-Cas9 system integrating genome-wide off-target prediction and detection

Poria cocos is an important edible and medicinal fungus with a long history. However, the lack of adequate genetic tools has hindered molecular genetic research and the genetic modification of this species. In this study, the endogenous U6 promoters were identified by mining data from the P. cocos g...

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出版年:	Frontiers in Microbiology
主要な著者:	Zhenni Xie, Can Zhong, Xiaoliu Liu, Ziling Wang, Rongrong Zhou, Jing Xie, Shuihan Zhang, Jian Jin
フォーマット:	論文
言語:	英語
出版事項:	Frontiers Media S.A. 2022-08-01
主題:	medicinal fungus Poria cocos CRISPR/Cas9 genome editing endogenous promoter off-target
オンライン･アクセス:	https://www.frontiersin.org/articles/10.3389/fmicb.2022.966231/full