Establishment of a genome editing tool using CRISPR-Cas9 ribonucleoprotein complexes in the non-model plant pathogen Sphaerulina musiva

CRISPR-Cas9 is a versatile genome editing system widely used since 2013 to introduce site-specific modifications into the genomes of model and non-model species. This technology is used in various applications, from gene knock-outs, knock-ins, and over-expressions to more precise changes, such as th...

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Bibliographic Details

Published in:	Frontiers in Genome Editing
Main Authors:	Joanna Tannous, Cole Sawyer, Md Mahmudul Hassan, Jesse L. Labbe, Carrie Eckert
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2023-07-01
Subjects:	CRISPR-Cas9 RNP spore-bearing fungi ascomycetes trichoderma reesei Sphaerulina musiva
Online Access:	https://www.frontiersin.org/articles/10.3389/fgeed.2023.1110279/full