Isolation method of brain microvessels from small frozen human brain tissue for blood-brain barrier protein expression analysis

Abstract Background Protein expression analysis of isolated brain microvessels provides valuable insights into the function of the blood-brain barrier (BBB). However, isolation of brain microvessels from human brain tissue, particularly in small quantities, poses significant challenges. This study p...

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Bibliographic Details

Published in:	Fluids and Barriers of the CNS
Main Authors:	Seiryo Ogata, Shingo Ito, Takeshi Masuda, Sumio Ohtsuki
Format:	Article
Language:	English
Published:	BMC 2024-12-01
Subjects:	Blood-brain barrier Human brain microvessel Quantitative protein expression profile Absolute protein expression Transporter
Online Access:	https://doi.org/10.1186/s12987-024-00609-6