Screening Method for CRISPR/Cas9 Inhibition of a Human DNA Virus: Herpes Simplex Virus

The efficiency of cleavage of individual CRISPR/Cas9-sgRNAs remains difficult to predict based on the CRISPR target sequence alone. Different intracellular environments (dependent on cell type or cell cycle state for example) may affect sgRNA efficiency by altering accessibility of genomic DNA throu...

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Bibliographic Details

Published in:	Bio-Protocol
Main Authors:	Werner Neuhausser, Hyung Oh, Pierce Eggan, Magdalena Angelova, Rory Kirchner, Kevin Eggan, David Knipe
Format:	Article
Language:	English
Published:	Bio-protocol LLC 2020-09-01
Online Access:	https://bio-protocol.org/en/bpdetail?id=3748&type=0