Protocol for quantifying muscle fiber size, number, and central nucleation of mouse skeletal muscle cross-sections using Myotally software

Summary: Here, we present a protocol for using Myotally, a user-friendly software for fast, automated quantification of muscle fiber size, number, and central nucleation from immunofluorescent stains of mouse skeletal muscle cross-sections. We describe steps for installing the software, preparing co...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Pieter Both, Soochi Kim, Jengmin Kang, Marina Arjona, Daniel I. Benjamin, Christopher W. Nutter, Armon Goshayeshi, Thomas A. Rando
Format:	Article
Language:	English
Published:	Elsevier 2025-03-01
Subjects:	Cell Biology Microscopy Computer sciences
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724007202